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KMID : 0545119950050030167
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 3 p.167 ~ p.171
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Isolation and Characterization of a Methylotroph Producing 3-hydroxybutyrate-3-hydroxyvalerate Copolymer
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Kim, Pil
Kim, Seon Won/Lee, Gyun Min/Lee, Hyun Soo/Kim, Jung Hoe
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Abstract
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A bacterial strain C-02 using methanol as a carbon source was isolated from Gumi Industrial Estate and selected based on its rapid growth and capability of poly-β-hydroxybutyrate accumulation. Characteristics of strain C-02 showed that it belongs to the Methylococcaceae family, Type Ⅱ subgroup. Strain C-02 could incorporate valerate into the PHB chain to form 3-hydroxybutyrate and 3-hydroxyvalerate (P(3HB-co-3HV)). Among various nutrient limitation tests, the nitrogen limitation test resulted in the highest content of P(3HB-co-3HV) per dry cell weight, 50%. Under the nitrogen limited condition, the average molecular weight of P(3HB-co-3HV) obtained was determined to be approximately 2.8×10 exp (5) daltons.
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